Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 20 Liters
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Alkali Scientific CellPro™ PBS Buffer, 1X , pH 7.4, 20L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
ILEX LIFE SCIENCES LLC PAN-Biotech Dulbecco's Phosphate-Buffered Saline (DPBS), w/o: Ca and Mg (500 ml)
DPBS, WITHOUT: Ca and Mg. QUANTITY: 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: Dulbecco's Phosphate-Buffered Saline (DPBS) without calcium chloride and magnesium chloride. The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment.
AdipoGen PBS Endotoxin-free (sterile) (100 ml)
PBS Endotoxin-free (sterile) (100 ml), AdipoGen Life Sciences. Chemical. Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Bioworld BSA (1%) IN PBS, DNASE AND RNA
BSA (1%) in PBS, DNase and RNase Free0.79% Sodium Chloride, 0.02% Potassium Chloride, 0.06% Sodium Phosphate, 0.02% Potassium Phosphate, 1.0% BSA, DNAse/RNAse-Free
Kd Medical Inc DPBS, 1X, W/O CA AND MG, 1L
Dulbecco's Phosphate Buffered Saline are recommended for use in various procedures used in biological and molecular research. Solution is without Calcium and Magnesium. One 1 Liter Bottle.
Kd Medical Inc PBS 1X, PH 7.4 W/SOD AZIDE,20L
Phosphate Buffered Saline is used as a wash buffer in several lab applications. Contains sodium azide FACS washing grade, pH 7.4. One 20 liter cubitainer.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
![Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10400-1000.0.JPG-250.jpg)
Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
PBS 1X Solution (Phosphate-Buffered Saline) is a balanced, isotonic buffer commonly used in biological and biomedical research. Prepared at a working concentration, it contains sodium chloride, sodium phosphate, and potassium phosphate to maintain pH and osmolarity. It is widely used in cell culture, molecular biology, and immunohistochemistry for cell washing, reagent preparation, and sample dilution. PBS helps preserve the integrity and viability of biological samples by providing a stable, non-disruptive environment. Its compatibility with most biological systems makes it a fundamental laboratory reagent.
Crystalgen Inc PBS (PHOSPHATE BUFFERED SALINE
PBS (Phosphate Buffered Saline) pH7.4, 10X, with Ca, Mg-4X1L
Biotium Blocking Buffers Background Reducers Fluorescence Microscopy Chemical Reagents TrueBlack 1/EA
This buffer is a component of the TrueBlack Background Suppressor System (Permeabilizing) It is an excellent general use non-mammalian blocking buffer with detergent for intracellular immunofluorescence Not recommended for use with HRP (contains azide) 100 mL bottle
Apexbio Technology LLC Annexin V-HF488/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes Propidium iodide (PI) selectively binds double-stranded DNA and yields a red fluorescence PI is membrane-impermeable in healthy cells but can penetrate the membranes of late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-HF488 and PI enables differentiation between early apoptotic late apoptotic and dead cells
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ PBS Buffer, with Ca + Mg, 1X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.