Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
S2 Media 100mL 1x Phosphate Buffered Saline (PBS), pH 7.4, 125mL Media Bottle, 1ea
1x Phosphate Buffered Saline (PBS), pH 7.4 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.
Research Products International Corp Bicine Ultra-Pure, 250 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Research Products International Corp TRIS Buffer Grade, 5 Kilograms
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Quality Biological Inc TBS 10X pH 7.4, 10 Liters
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
![Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-D22150-1000.0.JPG-250.jpg)
Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
Ready to use sterile 1x solution
Contains: 137 mM Sodium Chloride
2.7 mM Potassium Chloride
10 mM Phosphates
Does not contain Calcium or Magnesium
Quality Biological Inc TAE Buffer 50X, 10 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc DPBS without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Medchemexpress LLC Cetuximab PBS 5mg | 5mg
Cetuximab (PBS) is a human IgG1 monoclonal antibody that can inhibit EGFR The SPR method measured the Kd value of Cetuximab for EGFR to be 0 201 nM Cetuximab has a highly effective anti-tumor effect[1]
Research Products International Corp MES, Anhydrous, 25 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol